BbS2K-GFP-no-tetR (pCB060)
(Plasmid
#254870)
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PurposeBbS2K-GFP plasmid without tetR gene
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254870 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepBbS2K
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namesfGFP
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Insert Size (bp)720
- Promoter pTet
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.11.27.625634 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BbS2K-GFP-no-tetR (pCB060) was a gift from Mary Dunlop (Addgene plasmid # 254870 ; http://n2t.net/addgene:254870 ; RRID:Addgene_254870) -
For your References section:
Dynamic heterogeneity in an E. coli stress response regulon mediates gene activation and antimicrobial peptide tolerance. Blassick CM, Moghimianavval H, Jafarbeglou F, Lugagne JB, Dunlop MJ. Cell Rep. 2026 Apr 16;45(4):117259. doi: 10.1016/j.celrep.2026.117259. 10.1016/j.celrep.2026.117259 PubMed 41996246