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BbS2K-GFP-no-tetR (pCB060)
(Plasmid #254870)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254870 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pBbS2K
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    sfGFP
  • Insert Size (bp)
    720
  • Promoter pTet

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.11.27.625634 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BbS2K-GFP-no-tetR (pCB060) was a gift from Mary Dunlop (Addgene plasmid # 254870 ; http://n2t.net/addgene:254870 ; RRID:Addgene_254870)
  • For your References section:

    Dynamic heterogeneity in an E. coli stress response regulon mediates gene activation and antimicrobial peptide tolerance. Blassick CM, Moghimianavval H, Jafarbeglou F, Lugagne JB, Dunlop MJ. Cell Rep. 2026 Apr 16;45(4):117259. doi: 10.1016/j.celrep.2026.117259. 10.1016/j.celrep.2026.117259 PubMed 41996246