pMT-ef1a-h2b-mNeonGreen
(Plasmid
#254877)
-
PurposeThis plasmid is designed for Tol2-mediated transgenesis in zebrafish and drives ubiquitous nuclear tagged expression of mNeonGreen, under the control of an ef1a (eef1a1l1) derived promoter.
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254877 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepMT-ef1a-h2b-mCherry
-
Backbone manufacturerNicole Suren, Wagner Lab
- Total vector size (bp) 6200
-
Vector typeZebrafish Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namemNeonGreen
-
SpeciesSynthetic
-
Insert Size (bp)1400
- Promoter eef1a1l1
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T3
- 3′ sequencing primer T7
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pMT-ef1a-h2b-mNeonGreen was a gift from Dan Wagner (Addgene plasmid # 254877 ; http://n2t.net/addgene:254877 ; RRID:Addgene_254877)
