Lenti-CMV-GFP-sI-Arr3-HA
(Plasmid
#254889)
-
PurposeMammalian expression of arrestin-3
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254889 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepLemti6.4/R4R2/V5-DEST
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 8922
-
Vector typeLentiviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namearrestin-3
-
Alt nameb-arrestin2 isoform X1
-
SpeciesB. taurus (bovine)
-
Insert Size (bp)2650
-
GenBank IDXP_005220238.1
- Promoter CMV
-
Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Lenti-CMV-GFP-sI-Arr3-HA was a gift from Vsevolod Gurevich (Addgene plasmid # 254889 ; http://n2t.net/addgene:254889 ; RRID:Addgene_254889) -
For your References section:
Arrestin-3-assisted activation of JNK3 mediates dopaminergic behavioral sensitization. Ahmed MR, Zheng C, Dunning JL, Ahmed MS, Ge C, Pair FS, Gurevich VV, Gurevich EV. Cell Rep Med. 2024 Jul 16;5(7):101623. doi: 10.1016/j.xcrm.2024.101623. Epub 2024 Jun 26. 10.1016/j.xcrm.2024.101623 PubMed 38936368