Lenti-ENK-Venus
(Plasmid
#254896)
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PurposeMammalian expression of Venus
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254896 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepLemti6.4/R4R2/V5-DEST
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 8922
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVenus
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SpeciesAequorea victoria
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Insert Size (bp)740
- Promoter ENK
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
ENK is rat enkephalin promoter first described in Ferguson, et al. Nat Neurosci. 2011 (doi: https://doi.org/10.1038/nn.2703. PMID: 21131952).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Lenti-ENK-Venus was a gift from Vsevolod Gurevich (Addgene plasmid # 254896 ; http://n2t.net/addgene:254896 ; RRID:Addgene_254896) -
For your References section:
Arrestin-3-assisted activation of JNK3 mediates dopaminergic behavioral sensitization. Ahmed MR, Zheng C, Dunning JL, Ahmed MS, Ge C, Pair FS, Gurevich VV, Gurevich EV. Cell Rep Med. 2024 Jul 16;5(7):101623. doi: 10.1016/j.xcrm.2024.101623. Epub 2024 Jun 26. 10.1016/j.xcrm.2024.101623 PubMed 38936368