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pET28 GsMutY E43S
(Plasmid #254899)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254899 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET29A
  • Backbone size w/o insert (bp) 5371
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    N/A
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GsMutY E43S
  • Species
    Geobacillus stearothermophilus
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis tag

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2026.01.22.701053 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28 GsMutY E43S was a gift from Martin Horvath (Addgene plasmid # 254899 ; http://n2t.net/addgene:254899 ; RRID:Addgene_254899)

  • For your References section:

    Structural Basis for Nucleobase Activation by the Adenine DNA Glycosylase MutY. Russelburg LP, Demir M, Cedeno K, David SS, Horvath MP. bioRxiv [Preprint]. 2026 Jan 22:2026.01.22.701053. doi: 10.64898/2026.01.22.701053. 10.64898/2026.01.22.701053 PubMed 41648512
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