pcDNA3.1-HaloTagDisplay
(Plasmid
#254907)
-
PurposeExpresses HaloTag7 on the cell surface (type I membrane protein with HA tag and Myc tag)
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254907 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 6625
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameKozak-Start-Leader-HA-HaloTag7-MycTag-Transmembrane-Stop
-
SpeciesSynthetic
-
Insert Size (bp)1197
- Promoter CMV
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer T7
- 3′ sequencing primer BGH-rev
- (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byCommercial gene synthesis from BioCat, Heidelberg
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA3.1-HaloTagDisplay was a gift from Andreas Maurer (Addgene plasmid # 254907 ; http://n2t.net/addgene:254907 ; RRID:Addgene_254907) -
For your References section:
A PET reporter ligand for quantitative imaging of gene expression in the brain. Stotz S, Dunkel G, Haas S, Seyfried P, Kinzler J, Berner J, Schaller M, Lammerhofer M, Grant SGN, Pichler BJ, Weigelin B, Herfert K, Maurer A. Nat Biomed Eng. 2026 Jul 27. doi: 10.1038/s41551-026-01748-x. 10.1038/s41551-026-01748-x PubMed 42509348