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pET-26b_HyperTEV60/L1
(Plasmid #254925)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254925 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET-26b
  • Backbone size w/o insert (bp) 5234
  • Total vector size (bp) 697
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HyperTEV60/L1
  • Insert Size (bp)
    699
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • His-tag (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-26b_HyperTEV60/L1 was a gift from Mark Isalan (Addgene plasmid # 254925 ; http://n2t.net/addgene:254925 ; RRID:Addgene_254925)

  • For your References section:

    Loop engineering and activity improvement of TEV protease by a phagemid-based selection system. Yamaguchi H, Isalan M. Sci Rep (2026). doi: 10.1038/s41598-026-74102-5 10.1038/s41598-026-74102-5
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