pXJ520-pAAV-PB3'IR-CAG-MCP-GFP-KASH-6XMS2-hU6-gRNA-PB5'IR
(Plasmid
#254955)
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PurposeAAV piggyBac vector with single gRNA and CAG-driven MCP-sfGFP-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254955 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepAAV
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Vector typeMammalian Expression, AAV, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMCP-sfGFP-KASH
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Insert Size (bp)1395
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.64898/2026.03.15.711739 for the bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pXJ520-pAAV-PB3'IR-CAG-MCP-GFP-KASH-6XMS2-hU6-gRNA-PB5'IR was a gift from Xin Jin (Addgene plasmid # 254955 ; http://n2t.net/addgene:254955 ; RRID:Addgene_254955) -
For your References section:
In vivo multiomic Perturb-seq with enhanced nuclear gRNA capture. Zheng X, Li J, Kim K, Simmons SK, Zhao Z, Tastemel M, Huynh N, Qiu H, Ye J, White CM, Levin JZ, Jin X. bioRxiv [Preprint]. 2026 Mar 17:2026.03.15.711739. doi: 10.64898/2026.03.15.711739. 10.64898/2026.03.15.711739 PubMed 41889810
