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tetOFF-EGFP^PTC-35
(Plasmid #254992)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254992 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    XLone-tTA-MCS
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP^PTC-35
  • Species
    Synthetic
  • Mutation
    NA

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    tetOFF-EGFP^PTC-35 was a gift from Joshua Mendell (Addgene plasmid # 254992 ; http://n2t.net/addgene:254992 ; RRID:Addgene_254992)

  • For your References section:

    Ribosome Recycling by ABCE1 Links Lysosomal Function and Iron Homeostasis to 3' UTR-Directed Regulation and Nonsense-Mediated Decay. Zhu X, Zhang H, Mendell JT. Cell Rep. 2020 Jul 14;32(2):107895. doi: 10.1016/j.celrep.2020.107895. 10.1016/j.celrep.2020.107895 PubMed 32668236
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