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K18 Cys-free+HisTag
(Plasmid #255040)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255040 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET-28a(+)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    modified tau protein (K18)
  • Species
    H. sapiens (human)
  • Mutation
    A 4R K18 construct called K18 cysteine-free (K18CFh) with mutations at residues 291 and 322 cysteine to serine.
  • Entrez Gene
    MAPT (a.k.a. DDPAC, FTD1, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

We used a 4R K18 construct called K18 cysteine-free (K18CFh) with mutations at residues 291 and 322 cysteine to serine and a poly-histidine tag and a 3R K19 construct called K19 cysteine-free (K19CFh) with a mutation at residue 322 cysteine to serine and a poly-histidine tag.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    K18 Cys-free+HisTag was a gift from Byron Caughey (Addgene plasmid # 255040 ; http://n2t.net/addgene:255040 ; RRID:Addgene_255040)

  • For your References section:

    4-Repeat tau seeds and templating subtypes as brain and CSF biomarkers of frontotemporal lobar degeneration. Saijo E, Metrick MA 2nd, Koga S, Parchi P, Litvan I, Spina S, Boxer A, Rojas JC, Galasko D, Kraus A, Rossi M, Newell K, Zanusso G, Grinberg LT, Seeley WW, Ghetti B, Dickson DW, Caughey B. Acta Neuropathol. 2020 Jan;139(1):63-77. doi: 10.1007/s00401-019-02080-2. Epub 2019 Oct 16. 10.1007/s00401-019-02080-2 PubMed 31616982
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