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hamster FL PrP_pET-41a(+)
(Plasmid #255046)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255046 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET-41a(+)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    full length hamster prion protein (aa 23-231)
  • Species
    Syrian Golden Hamster
  • Mutation
    Residues 23-231; accession K02234.
  • Entrez Gene
    Prnp (a.k.a. PRP, PrP27-30, PrP33-35C)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

DNA Sequences coding for hamster (residues 23-231; accession K02234) rPrPc residues were amplified and ligated into the pET41 vector (EMD Biosciences) and sequences were verified. Plasmids were transformed into E. coli Rosetta cells (EMD Biosciences).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hamster FL PrP_pET-41a(+) was a gift from Byron Caughey (Addgene plasmid # 255046 ; http://n2t.net/addgene:255046 ; RRID:Addgene_255046)

  • For your References section:

    Rapid end-point quantitation of prion seeding activity with sensitivity comparable to bioassays. Wilham JM, Orru CD, Bessen RA, Atarashi R, Sano K, Race B, Meade-White KD, Taubner LM, Timmes A, Caughey B. PLoS Pathog. 2010 Dec 2;6(12):e1001217. doi: 10.1371/journal.ppat.1001217. 10.1371/journal.ppat.1001217 PubMed 21152012
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