TDP43 263D_pET-28a(+)
(Plasmid #255047)

• Purpose: Recombinant TDP43 construct for seeding activity assays and other applications.
• Depositing Lab: Byron Caughey
• Publication: Rational Generation of Monoclonal Antibodies and Intrabodies Selective for Pathogenic TDP-43 (unpublished)

Backbone

• Vector backbone: pET-28a(+)
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: TAR DNA-binding protein 43 from 263-414 amino acids without any mutations.
• Species: H. sapiens (human)
• Mutation: 263D
• Entrez Gene: TARDBP (a.k.a. ALS10, TDP-43)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry