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pET28-HLA-A03-AviHis
(Plasmid #255049)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255049 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET28a
  • Total vector size (bp) 6146
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use BL21(DE3) for protein expression
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HLA-A01:03 extracellular region
  • Species
    H. sapiens (human)
  • Promoter T7
  • Tag / Fusion Protein
    • Avi tag and his tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7promoter
  • 3′ sequencing primer T7terminator
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-HLA-A03-AviHis was a gift from Shohei Koide (Addgene plasmid # 255049 ; http://n2t.net/addgene:255049 ; RRID:Addgene_255049)

  • For your References section:

    Creating MHC-Restricted Neoantigens with Covalent Inhibitors That Can Be Targeted by Immune Therapy. Hattori T, Maso L, Araki KY, Koide A, Hayman J, Akkapeddi P, Bang I, Neel BG, Koide S. Cancer Discov. 2023 Jan 9;13(1):132-145. doi: 10.1158/2159-8290.CD-22-1074. 10.1158/2159-8290.CD-22-1074 PubMed 36250888
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