Skip to main content
Addgene
Open Menu

pHBT-Mb(NRAS_24)
(Plasmid #255052)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255052 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart

Backbone

  • Vector backbone
    pET28a
  • Total vector size (bp) 5623
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use BL21(DE3) for protein expression
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Mb(NRAS_24)
  • Species
    Synthetic
  • Insert Size (bp)
    381
  • Promoter T7
  • Tag / Fusion Protein
    • His tag, Avi tag, TEV cleavage site (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7promoter
  • 3′ sequencing primer T7terminator
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHBT-Mb(NRAS_24) was a gift from Shohei Koide (Addgene plasmid # 255052 ; http://n2t.net/addgene:255052 ; RRID:Addgene_255052)

  • For your References section:

    Inhibition and degradation of NRAS with a pan-NRAS monobody. Whaby M, Ketavarapu G, Koide A, Mazzei M, Mintoo M, Glasser E, Patel U, Nasarre C, Sale MJ, McCormick F, Koide S, O'Bryan JP. Oncogene. 2024 Nov;43(48):3489-3497. doi: 10.1038/s41388-024-03186-y. Epub 2024 Oct 8. 10.1038/s41388-024-03186-y PubMed 39379700
Related items: