pGEX - ING3
(Plasmid
#255113)
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PurposeProtein expression of the ING3 PHD domain
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 255113 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepGEX-4T-1
- Backbone size w/o insert (bp) 4900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameING3
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SpeciesH. sapiens (human)
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MutationNone
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Entrez GeneING3 (a.k.a. Eaf4, ING2, MEAF4, p47ING3)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GenBank ID Q9NXR8
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX - ING3 was a gift from Mark Bedford (Addgene plasmid # 255113 ; http://n2t.net/addgene:255113 ; RRID:Addgene_255113) -
For your References section:
Characterization of the plant homeodomain (PHD) reader family for their histone tail interactions. Jain K, Fraser CS, Marunde MR, Parker MM, Sagum C, Burg JM, Hall N, Popova IK, Rodriguez KL, Vaidya A, Krajewski K, Keogh MC, Bedford MT, Strahl BD. Epigenetics Chromatin. 2020 Jan 24;13(1):3. doi: 10.1186/s13072-020-0328-z. 10.1186/s13072-020-0328-z PubMed 31980037