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SP-HZ
(Plasmid #255264)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255264 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pBT13
  • Backbone manufacturer
    David R. Liu
  • Backbone size w/o insert (bp) 7326
  • Total vector size (bp) 7607
  • Modifications to backbone
    T7 Promoter upstream of GOI Spacer region between GOI and T7 Terminator T7 Terminator downstream of spacer region
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HinZip (GOI)
  • Alt name
    HZ
  • Species
    Synthetic
  • Insert Size (bp)
    285
  • Promoter Phage promoter
  • Tag / Fusion Protein
    • RNAP Omega Subunit (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CTCGTCAGATGCAGGTAG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Selection Phage backbone constructed by combining Addgene#122597 and Addgene#122596

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SP-HZ was a gift from Jumi Shin (Addgene plasmid # 255264 ; http://n2t.net/addgene:255264 ; RRID:Addgene_255264)

  • For your References section:

    Unlocking genetic potential: harnessing phage for targeted mutagenesis in phage-assisted evolution. Ali M, Khan A, Nursimulu TV, Shin JA. Nucleic Acids Res. 2025 Jul 19;53(14):gkaf746. doi: 10.1093/nar/gkaf746. 10.1093/nar/gkaf746 PubMed 40794866
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