ks-99-backbone
(Plasmid
#255270)
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Purpose(Empty Backbone) Serves as an empty vector control for LRE3 overexpression from ks-99-GFP-LRE3-sv(+)
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 255270 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneks-99-backbone
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Backbone manufacturerBenayoun lab
- Backbone size (bp) 2895
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Modifications to backboneInsert from ks-99-GFP-LRE3-sv(+) plasmid gifted by Dr. John Moran was removed by restriction digest to create a control backbone from transfection. Original plasmid described in https://academic.oup.com/hmg/article-abstract/16/13/1569/2355984
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Other
Resource Information
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A portion of this plasmid was derived from a plasmid made byInsert from ks-99-GFP-LRE3-sv(+) plasmid gifted by Dr. John Moran was removed by restriction digest to create a control backbone from transfection. Original plasmid described in https://academic.oup.com/hmg/article-abstract/16/13/1569/2355984
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ks-99-backbone was a gift from Bérénice Benayoun (Addgene plasmid # 255270 ; http://n2t.net/addgene:255270 ; RRID:Addgene_255270)