pcDNA3.1(+)_L1RP_ORF2-only
(Plasmid
#255274)
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PurposeOverexpress L1RP's ORF2 using the high expression CMV promoter.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 255274 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepcDNA3.1(+)
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5428
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameL1RP_ORF2-only
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SpeciesH. sapiens (human)
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Insert Size (bp)3828
- Promoter CMV
Cloning Information
- Cloning method Other
- 5′ sequencing primer CMV-F
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byORF2 sequence was amplified from pDA077 (Plasmid #131388)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1(+)_L1RP_ORF2-only was a gift from Bérénice Benayoun (Addgene plasmid # 255274 ; http://n2t.net/addgene:255274 ; RRID:Addgene_255274)