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pENTR-D-sp6-cfk-kcnh8
(Plasmid #255313)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255313 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pENTR-D-sp6
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2606
  • Total vector size (bp) 5615
  • Modifications to backbone
    Sp6 was introduced between M13F and attL1
  • Vector type
    Mammalian Expression, Gateway Cloning ; In vitro transcription for riboprobes

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    kcnh8
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    3009
  • Mutation
    Stop codon removed
  • GenBank ID
  • Entrez Gene
    kcnh8 (a.k.a. si:ch211-195k18.3)
  • Promoter T7 and Sp6

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-D-sp6-cfk-kcnh8 was a gift from GuangJun Zhang (Addgene plasmid # 255313 ; http://n2t.net/addgene:255313 ; RRID:Addgene_255313)

  • For your References section:

    Evolutionary Analysis of Vertebrate KCNH Voltage-Gated Potassium Channels and Spatial Expression of kcnh Genes in Zebrafish Embryos. Wu K, Wang D, Dong Z, Zhou AY, Zhang G. Journal of Developmental Biology. 2026; 14(3):32. 10.3390/jdb14030032
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