pT7_EcDRT3_3a-IntHis6
(Plasmid
#255317)
-
PurposeExpresses EcDRT3 from Escherichia coli GF3-3 with an internal His6 tag on Drt3a
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 255317 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepT7
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameEcDRT3 (Drt3a + Drt3b + ncRNA) with an internal His6 tag between Drt3a Thr247 and Glu248
-
Tag
/ Fusion Protein
- His6
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pT7_EcDRT3_3a-IntHis6 was a gift from Alex Gao (Addgene plasmid # 255317 ; http://n2t.net/addgene:255317 ; RRID:Addgene_255317) -
For your References section:
Protein-templated synthesis of dinucleotide repeat DNA by an antiphage reverse transcriptase. Deng P, Lee H, Armijo C, Wang H, Gao A. Science. 2026 Apr 16:eaed1656. doi: 10.1126/science.aed1656. 10.1126/science.aed1656 PubMed 41990131