CSII-CMV-mRFP1-IRES2-BsdR
(Plasmid
#255330)
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PurposeLentiviral expression of mRFP1 and BsdR (via IRES2) under the CMV promoter
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 255330 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneCSII-CMV-MCS-IRES2-Bsd
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Backbone manufacturerRIKEN BRC
- Backbone size w/o insert (bp) 9545
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert namemonomeric red fluorescent protein 1
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Alt namemRFP1
Gene/Insert 2
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Gene/Insert nameblasticidin resistance gene
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Alt nameBsdR
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.06.23.661005 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CSII-CMV-mRFP1-IRES2-BsdR was a gift from Yuichiro Miyaoka (Addgene plasmid # 255330 ; http://n2t.net/addgene:255330 ; RRID:Addgene_255330)