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CSII-EF1alpha-H2BC11-VENUS-IRES2-PuroR
(Plasmid #255331)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255331 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    CSII-EF-GFP-IRES-PuroR
  • Backbone manufacturer
    RIKEN BRC
  • Backbone size w/o insert (bp) 11482
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    Histone 2BC11-fused VENUS
  • Alt name
    H2BC11-VENUS

Gene/Insert 2

  • Gene/Insert name
    Puromycin resistant gene
  • Alt name
    PuroR

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.06.23.661005 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CSII-EF1alpha-H2BC11-VENUS-IRES2-PuroR was a gift from Yuichiro Miyaoka (Addgene plasmid # 255331 ; http://n2t.net/addgene:255331 ; RRID:Addgene_255331)
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