pAL250 :: msfGFP
(Plasmid
#255423)
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PurposeContains 250 bp region of Pxyl promoter expressing msfGFP; insensitive to xylose.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 255423 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepAL250
- Backbone size w/o insert (bp) 8169
- Total vector size (bp) 8890
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Modifications to backbonepAL250 is pTA962 with 250 bp from the pXyl promoter added
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Vector typeHaloarchaeal expression
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Selectable markerspyrE2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMonomeric Superfolder Green Fluorescent Protein
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Alt namemsfGFP
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Insert Size (bp)721
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAL250 :: msfGFP was a gift from Alexandre Bisson (Addgene plasmid # 255423 ; http://n2t.net/addgene:255423 ; RRID:Addgene_255423) -
For your References section:
A sweet new set of inducible and constitutive promoters in Haloferax volcanii. Rados T, Andre K, Cerletti M, Bisson A. Front Microbiol. 2023 Aug 10;14:1204876. doi: 10.3389/fmicb.2023.1204876. eCollection 2023. 10.3389/fmicb.2023.1204876 PubMed 37637112