pPHYCUT
(Plasmid
#255426)
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Purpose(Empty Backbone) Expresses codon-optimized versions of SpCas9 and Csy4, with a U6 promoter-driven BsaI cloning casette for the expression of multiple sgRNAs in Phaeodactylum tricornutum.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 255426 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepPtGE34
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Backbone manufacturerDavid Edgell
- Backbone size (bp) 13278
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Modifications to backboneE. coli origin of replication and selection marker swapped to p15A origin and chloramphenicol resistance. Replacement of SpCas9 promoter with P. tricornutum nitrate reductase promoter. Addition of codon-optimized Csy4 gene, driven by P. tricornutum H4-1β promoter and terminator.
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Vector typeCRISPR, Synthetic Biology ; Episomal vector for Phaeodactylum tricornutum
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Selectable markersZeocin, HIS3
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsEPI300 growth strain also recommended.
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Copy numberLow Copy
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPHYCUT was a gift from David Edgell (Addgene plasmid # 255426 ; http://n2t.net/addgene:255426 ; RRID:Addgene_255426)
Map uploaded by the depositor.
