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AAV-S5E2-jGCaMP8f-WPRE
(Plasmid #255452)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255452 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pAAV-S5E2-GCaMP6f
  • Backbone manufacturer
    Addgene #135632
  • Backbone size w/o insert (bp) 4698
  • Total vector size (bp) 6051
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    jGCaMP8f
  • Species
    R. norvegicus (rat), G. gallus (chicken); A. victoria (jellyfish)
  • Insert Size (bp)
    1355
  • Promoter S5E2
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (1125) (destroyed during cloning)
  • 3′ cloning site HindIII (2473); [NcoI (1090)-NheI (1125) blunt ligation] (unknown if destroyed)
  • 5′ sequencing primer pBR322ori-F
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    jGCaMP8f

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-S5E2-jGCaMP8f-WPRE was a gift from Eric Melonakos (Addgene plasmid # 255452 ; http://n2t.net/addgene:255452 ; RRID:Addgene_255452)
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