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pET15b_6xHis_mScarlet-I
(Plasmid #255536)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255536 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET15b
  • Total vector size (bp) 6552
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mScarlet-I
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHIS and thrombin site (C terminal on insert)
    • FLAG, tetracysteine, and dBroccoli (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Cloned by Simon J. Moore

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b_6xHis_mScarlet-I was a gift from Simon Moore (Addgene plasmid # 255536 ; http://n2t.net/addgene:255536 ; RRID:Addgene_255536)

  • For your References section:

    A liquid handling platform for standardised quantification of cell-free enzymatic activity encoded by antimicrobial resistance genes. Bergum M, Suthakaran S, Martin B, Sutton JM, Moore SJ. Synthetic Biology, 2026;, ysag010, doi: 10.1093/synbio/ysag010 10.1093/synbio/ysag010
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