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CAG-loxp-stop-loxp-IFNgR1-QY-tTA-T2A-IFNgR2-TEV-IRES-TagBFP-PA
(Plasmid #255644)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255644 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pGEM3Z
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    IfngR1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Ifngr1 (a.k.a. CD119, IFN-gammaR, Ifgr, Ifngr, Nktar)
  • Tag / Fusion Protein
    • tTA (C terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    ifngr2
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Ifngr2 (a.k.a. Ifgr2, Ifgt)
  • Tag / Fusion Protein
    • TEV (C terminal on insert)

Gene/Insert 3

  • Gene/Insert name
    TagBFP

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CAG-loxp-stop-loxp-IFNgR1-QY-tTA-T2A-IFNgR2-TEV-IRES-TagBFP-PA was a gift from Jun Huh (Addgene plasmid # 255644 ; http://n2t.net/addgene:255644 ; RRID:Addgene_255644)

  • For your References section:

    In vivo detection of immune responses via cytokine activity labeling. Lu G, Zhang S, Feng M, Kim E, Cho D, Kim JH, Caris H, Silberstein L, Choi GB, Huh JR. Cell. 2026 Feb 5;189(3):939-955.e26. doi: 10.1016/j.cell.2025.12.011. Epub 2026 Jan 7. 10.1016/j.cell.2025.12.011 PubMed 41506266
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