pLEX307-G418-T7pol
(Plasmid
#255668)
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PurposeConstitutively expresses T7 RNA polymerase under EF-1alpha promoter with G418 resistance marker.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 255668 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepLEX307-G418
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Backbone manufacturerBackbone is plex_307-ccdB-g418 (Addgene plasmid #201141, Alejandro Chavez Lab).
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Vector typeMammalian Expression, Lentiviral, Gateway Cloning
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameT7 RNA polymerase
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SpeciesBacteriophage T7
Resource Information
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A portion of this plasmid was derived from a plasmid made byT7 RNA polymerase gene is from pCAG-T7pol (Addgene plasmid #59926, Ian Wickersham Lab).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLEX307-G418-T7pol was a gift from Sho Iketani (Addgene plasmid # 255668 ; http://n2t.net/addgene:255668 ; RRID:Addgene_255668)