pAAV_TRE_HA_APOBEC1_mRPL10A_hSyn_rtTAadvanced_WPRE249
(Plasmid
#255698)
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PurposeInducible neuron-specific AAV Ribo-STAMP expression, mRPL10A_APOBEC1
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 255698 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepAAV_TRE_HA_APOBEC1_mRPL10A_hSyn_rtTAadvanced_WPRE249
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Backbone manufacturerVectorBuilder
- Total vector size (bp) 6468
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRPL10A
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SpeciesM. musculus (mouse)
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Insert Size (bp)648
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Entrez GeneRpl10a (a.k.a. CsA-19, Nedd6)
- Promoter TRE promoter
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Tag
/ Fusion Protein
- HA_APOBEC1
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer TET-F
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV_TRE_HA_APOBEC1_mRPL10A_hSyn_rtTAadvanced_WPRE249 was a gift from Eugene Yeo (Addgene plasmid # 255698 ; http://n2t.net/addgene:255698 ; RRID:Addgene_255698) -
For your References section:
Single-cell and isoform-specific translational profiling of the mouse brain. Sison SL, Zampa F, Kofman ER, Choi SY, Jagannatha P, Nguyen GG, Naritomi JT, Shin A, Khorgade A, Jin W, Chen CY, Sievert DM, Mukhopadhyay S, Mizrahi O, Blue SM, Marina RJ, Yang D, Wang CC, Pang Z, Brannan KW, Ye L, Al'Khafaji AM, Yeo GW, Lippi G. Nature. 2026 Feb 18. doi: 10.1038/s41586-026-10118-1. 10.1038/s41586-026-10118-1 PubMed 41708856