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pAAV-CW3SL-GCaMP7f-p2a-4Ala-H2B-PAmCherry
(Plasmid #255747)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255747 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pAAV
  • Backbone manufacturer
    Addgene #133419
  • Total vector size (bp) 6300
  • Modifications to backbone
    The CAG promoter was substituted with the CaMKII promoter, and the SV40-bGH-PolyA signal was replaced by WPRE-SV40-PolyA.
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    jGCaMP7f
  • Species
    Synthetic
  • Insert Size (bp)
    1350
  • Promoter CaMKII

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    H2B
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    378
  • Entrez Gene
    H2B
  • Entrez Gene
    H2BC11 (a.k.a. H2B/r, H2BFR, H2BJ, HIST1H2BJ)
  • Tags / Fusion Proteins
    • PAmCherry (C terminal on insert)
    • 4ALA (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CW3SL-GCaMP7f-p2a-4Ala-H2B-PAmCherry was a gift from Ivo Spiegel (Addgene plasmid # 255747 ; http://n2t.net/addgene:255747 ; RRID:Addgene_255747)

  • For your References section:

    2P-NucTag: On-demand phototagging for molecular analysis of functionally identified cortical neurons. Shi J, Nutkovich B, Kushinsky D, Rao BY, Herrlinger SA, Tsivourakis E, Mihaila TS, Conde Paredes ME, Cohen-Kashi Malina K, O'Toole CK, Yong HC, Sanner BM, Xie A, Varol E, Losonczy A, Spiegel I. Neuron. 2026 Apr 2:S0896-6273(26)00180-7. doi: 10.1016/j.neuron.2026.03.016. 10.1016/j.neuron.2026.03.016 PubMed 41932327
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