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pAAV-hSyn-Flex-Kir2.2mut-t2a-mRuby3
(Plasmid #255749)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255749 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pAAV
  • Backbone manufacturer
    Addgene #51505
  • Total vector size (bp) 6589
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    KCNJ12
  • Alt name
    Kir2.2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2861
  • Mutation
    GYG to AAA (aa145-147)
  • Entrez Gene
    Kcnj12 (a.k.a. IRK-2, IRK2, Kir2.2, MB-IRK2)
  • Promoter hSyn

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    mRuby
  • Species
    Synthetic
  • Insert Size (bp)
    711

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hSyn-Flex-Kir2.2mut-t2a-mRuby3 was a gift from Ivo Spiegel (Addgene plasmid # 255749 ; http://n2t.net/addgene:255749 ; RRID:Addgene_255749)

  • For your References section:

    2P-NucTag: On-demand phototagging for molecular analysis of functionally identified cortical neurons. Shi J, Nutkovich B, Kushinsky D, Rao BY, Herrlinger SA, Tsivourakis E, Mihaila TS, Conde Paredes ME, Cohen-Kashi Malina K, O'Toole CK, Yong HC, Sanner BM, Xie A, Varol E, Losonczy A, Spiegel I. Neuron. 2026 Apr 2:S0896-6273(26)00180-7. doi: 10.1016/j.neuron.2026.03.016. 10.1016/j.neuron.2026.03.016 PubMed 41932327
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