phSyn-Xph20-eGFP-CCR5TC
(Plasmid
#255756)
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Purposep-ZFB-Syn-Xph20-eGFP-CCR5TC: PSD-95 intrabody (Xph20) fused to eGFP with a CCR5 zinc finger for labelling the postsynaptic density.
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Depositing Lab
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Depositing OrganizationUniversitaetsklinikum Hamburg-Eppendorf
Located in Germany -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 255756 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCAG
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Backbone manufacturerDon B. Arnold lab (USC)
- Backbone size w/o insert (bp) 4619
- Total vector size (bp) 6234
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameXph20
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SpeciesSynthetic
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Insert Size (bp)1615
- Promoter hSyn
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site MluI (not destroyed)
- 5′ sequencing primer CTTCCCGGCCACCTTGGTC
- 3′ sequencing primer CTTCCCGGCCACCTTGGTC
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byA portion of this plasmid was derived from pCAG_Xph20-eGFP-CCR5TC (Addgene plasmid #135530), a gift from Matthieu Sainlos.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.64898/2026.04.09.717511 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
phSyn-Xph20-eGFP-CCR5TC was a gift from Thomas Oertner (Addgene plasmid # 255756 ; http://n2t.net/addgene:255756 ; RRID:Addgene_255756)