ELP-KQ30 with tyrosinase sites
(Plasmid
#255776)
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PurposeExpresses ELP-KQ30 with tyrosinase crosslinking sites at the N-terminus
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 255776 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepET-24a(+)
- Backbone size w/o insert (bp) 5242
- Total vector size (bp) 5767
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameELP-KQ30 with tyrosinase sites
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Alt nameElastin-like polypeptide with alternating lysine and glutamine guest residues
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SpeciesSynthetic
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Insert Size (bp)525
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Tag
/ Fusion Protein
- 6xHis (N terminal on insert)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ELP-KQ30 with tyrosinase sites was a gift from Allie Obermeyer (Addgene plasmid # 255776 ; http://n2t.net/addgene:255776 ; RRID:Addgene_255776) -
For your References section:
Biomimetic Approach to the Formation of Protein Materials via Complex Coacervation of Engineered Polypeptides. Fisher RS, Cheng Y, Ling NR, Goessling L, Obermeyer AC. ACS Synth Biol. 2026 Feb 20;15(2):472-480. doi: 10.1021/acssynbio.5c00592. Epub 2026 Jan 21. 10.1021/acssynbio.5c00592 PubMed 41565596