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pCvL31-mNeonGreen
(Plasmid #255779)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255779 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pBluescript
  • Backbone size w/o insert (bp) 2931
  • Total vector size (bp) 5292
  • Vector type
    Bacterial Expression ; Chromera velia

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mNeonGreen
  • Insert Size (bp)
    705
  • Promoter putative C. velia L31 promoter (1103 bp)
  • Tag / Fusion Protein
    • GGTGSGGTGSGGS (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer pBluescript KS
  • 3′ sequencing primer pBluescript SK
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pBluescript-derived expression vector for Chromera velia. A synthesized DNA fragment containing the putative C. velia L31 promoter region (1103 bp; VEuPathDB: Cvel_7598), mNeonGreen CDS, a 13-aa linker, and the C. velia L31 3′ UTR (499 bp) was ligated into the HindIII and BamHI sites of pBluescript. The putative L31 promoter is intended to drive reporter expression in C. velia.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCvL31-mNeonGreen was a gift from Ke Hu (Addgene plasmid # 255779 ; http://n2t.net/addgene:255779 ; RRID:Addgene_255779)

  • For your References section:

    Transfection of the free-living alga Chromera velia enables direct comparisons with its parasitic apicomplexan relative, Toxoplasma gondii. Tengganu IF, Hu K. J Cell Sci. 2026 Jan 1;139(1):jcs.264400. doi: 10.1242/jcs.264400. Epub 2026 Jan 13. 10.1242/jcs.264400 PubMed 41208444
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