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pCvGAPDH-CvH2B-mStayGold
(Plasmid #255783)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255783 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pBluescript
  • Backbone size w/o insert (bp) 2931
  • Total vector size (bp) 5850
  • Vector type
    Bacterial Expression ; Chromera velia, Toxoplasma gondii

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Chromera velia histone H2B (VEupathDB: Cvel_19200)
  • Alt name
    CvH2B
  • Insert Size (bp)
    351
  • Promoter putative C. velia GAPDH promoter (1090 bp)
  • Tag / Fusion Protein
    • mStayGold (codon-optimized for Chromera) (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer pBluescript KS
  • 3′ sequencing primer pBluescript SK
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Derived from pCvGAPDH-NanoLuc^opt, a pBluescript-based vector containing the Chromera velia GAPDH promoter (1090 bp; VEuPathDB: Cvel_5775), the first intron of C. velia β-tubulin (106 bp; VEuPathDB: Cvel_33153), the GAPDH Kozak sequence, a NanoLuc CDS codon-optimized for C. velia, and CvGAPDH 3'UTR (632 bp). Backbone was generated by digestion with PstI, which cuts immediately upstream of the Kozak sequence, and AflII, which cuts immediately downstream of the NanoLuc stop codon.

A gBlock fragment that includes the Kozak sequence, CDS of Chromera velia histone H2B (351 bp; VEupathDB: Cvel_19200) including the NLS, a 13-aa linker, and mStayGold codon-optimized for Chromera (675 bp), with both ends containing ~20-bp sequence that overlaps with the backbone insertion sites was synthesized (IDT Inc.) and inserted into the PstI and AflII sites of pCvGAPDH-NanoLuc^opt using Gibson assembly.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCvGAPDH-CvH2B-mStayGold was a gift from Ke Hu (Addgene plasmid # 255783 ; http://n2t.net/addgene:255783 ; RRID:Addgene_255783)

  • For your References section:

    Transfection of the free-living alga Chromera velia enables direct comparisons with its parasitic apicomplexan relative, Toxoplasma gondii. Tengganu IF, Hu K. J Cell Sci. 2026 Jan 1;139(1):jcs.264400. doi: 10.1242/jcs.264400. Epub 2026 Jan 13. 10.1242/jcs.264400 PubMed 41208444
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