sgAAVS1_1 in PspCas13b-NES-lentiCRISPRv2-PuroR
(Plasmid
#255846)
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PurposesgAAVS1_1 (Cas9) and PspCa13b-NES expression
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 255846 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepRDA221 no Puro
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAAVS1
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gRNA/shRNA sequenceGTGGTTGATAAACCCACGTG
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SpeciesH. sapiens (human)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
sgAAVS1_1 in PspCas13b-NES-lentiCRISPRv2-PuroR was a gift from Sefi Rosenbluh (Addgene plasmid # 255846 ; http://n2t.net/addgene:255846 ; RRID:Addgene_255846)