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C-mCherry-TMEM230
(Plasmid #255857)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255857 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5349
  • Total vector size (bp) 6516
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TMEM230
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    360
  • Entrez Gene
    TMEM230 (a.k.a. C20orf30, HSPC274, dJ1116H23.2.1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • C-mCherry with 3C cleavage site (C terminal on insert)
    • Flag-tag (C terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gene TMEM230 was codon-optimised for human expression and de novo synthesised by GENEWIZ from Azenta Life Science.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    C-mCherry-TMEM230 was a gift from Joseph Lyons (Addgene plasmid # 255857 ; http://n2t.net/addgene:255857 ; RRID:Addgene_255857)

  • For your References section:

    Fluorescence-Gated Flow Cytometry Approach for Measuring Lipid Flippase Activity in Mammalian Cells. Scholtissek KT, Van den Haute C, Pamula FK, Lyons JA. J Membrane Biol 259, 32 (2026) 10.1007/s00232-026-00392-5
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