pCMV-HYlight2-dead
(Plasmid
#255887)
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PurposeExpresses a binding dead mutant of HYlight2 cytosolically in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 255887 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 4200
- Total vector size (bp) 5714
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHYlight2-dead
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SpeciesSynthetic
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Insert Size (bp)1515
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MutationT67E
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.64898/2026.04.29.721630 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-HYlight2-dead was a gift from Alison Tebo (Addgene plasmid # 255887 ; http://n2t.net/addgene:255887 ; RRID:Addgene_255887) -
For your References section:
Improved sensors for fructose-1,6-bisphosphate enable in vivo imaging of glycolysis. Tyler J, Vishwanath AA, Menon T, Duarah T, Adhikari R, Koberstein JN, Feliciano D, Espinosa-Medina I, Colón-Ramos D, Tebo AG. bioRxiv 2026.04.29.721630 10.64898/2026.04.29.721630