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TRE3G-BI-NGN1-ISL1-Puro
(Plasmid #255892)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255892 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pUCM
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    EPI300
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    ISL1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1050
  • Entrez Gene
    ISL1 (a.k.a. ISLET1, Isl-1)
  • Promoter TRE3G BI

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Ngn1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    735
  • Mutation
    codon optimized
  • Entrez Gene
    Neurog1 (a.k.a. AKA, Math4C, Neurod3, bHLHa6, ngn1)
  • Promoter TREG BI

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TRE3G-BI-NGN1-ISL1-Puro was a gift from Samuele Marro (Addgene plasmid # 255892 ; http://n2t.net/addgene:255892 ; RRID:Addgene_255892)

  • For your References section:

    Induction of human pruriceptors from pluripotent stem cells via transcription factors. Iriki H, Hu R, Li X, Baljinnyam E, Habich C, Imanishi I, Miller LN, Chegireddy K, Malik LI, Yassky D, Kubler R, Heul AV, Smith KM, Reinhardt P, Goedken ER, Kim BS, Marro SG. Stem Cell Reports. 2026 Jul 9:103004. doi: 10.1016/j.stemcr.2026.103004. 10.1016/j.stemcr.2026.103004 PubMed 42425092
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