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NFAT_4X
(Plasmid #255904)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255904 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pTwist Amp High Copy
  • Backbone size w/o insert (bp) 3442
  • Total vector size (bp) 3845
  • Modifications to backbone
    None
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    4xNFAT-RE, NLuc-hPEST
  • Alt name
    NFAT
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    403
  • Promoter NFAT (IL-2)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Promega, UK

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NFAT_4X was a gift from Graham Ladds (Addgene plasmid # 255904 ; http://n2t.net/addgene:255904 ; RRID:Addgene_255904)

  • For your References section:

    Enhancement of a nuclear factor of activated T cells (NFAT) reporter for the study of G protein-coupled receptors. Wills E, Saji A, Sumner J, Khan A, Sisk CM, Shehata M, Taylor B, Ladds G. Commun Biol. 2026 Apr 26. doi: 10.1038/s42003-026-10110-5. 10.1038/s42003-026-10110-5 PubMed 42034830
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