Skip to main content
Addgene
Open Menu

pTR01F_hFGFR3c-A391E-V5
(Plasmid #255932)

Print
  • Purpose
    for PiggyBac mediated integration and stable expression of hFGFR3c-N540K protein, mutant variant causes ligand independent receptor activation and Crouzon syndrom with acanthosis nigricans
  • Depositing Lab
    Pavel Krejčí
  • Depositing Organization
    Masaryk University
    Located in Czech Republic
  • Publication
    Krejci Lab plasmids (unpublished) ( How to cite )
  • Sequence Information

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255932 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart

Backbone

  • Vector backbone
    TR01F
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human FGFR3c-A391E
  • Species
    H. sapiens (human)
  • Mutation
    A391E substitution
  • Promoter truncated CMV promoter
  • Tag / Fusion Protein
    • V5/His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The TR01F plasmid was obtained as a gift from Valeri Mossine (PMID: 24376882)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTR01F_hFGFR3c-A391E-V5 was a gift from Pavel Krejčí (Addgene plasmid # 255932 ; http://n2t.net/addgene:255932 ; RRID:Addgene_255932)
Related items: