pTR01F_hFGFR3c-A391E-V5
(Plasmid
#255932)
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Purposefor PiggyBac mediated integration and stable expression of hFGFR3c-N540K protein, mutant variant causes ligand independent receptor activation and Crouzon syndrom with acanthosis nigricans
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 255932 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneTR01F
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman FGFR3c-A391E
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SpeciesH. sapiens (human)
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MutationA391E substitution
- Promoter truncated CMV promoter
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Tag
/ Fusion Protein
- V5/His (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The TR01F plasmid was obtained as a gift from Valeri Mossine (PMID: 24376882)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTR01F_hFGFR3c-A391E-V5 was a gift from Pavel Krejčí (Addgene plasmid # 255932 ; http://n2t.net/addgene:255932 ; RRID:Addgene_255932)
