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pT.cruzi-RBP-MASP_6xHis
(Plasmid #256075)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 256075 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET30a
  • Backbone size w/o insert (bp) 5422
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use BL21 (DE3) for expression
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RNA-binding protein, mucin-associated surface protein (MASP)
  • Alt name
    TcBrA4_0104680 +TcBrA4_0101980
  • Alt name
    Tc3
  • Species
    Trypanosoma cruzi
  • Insert Size (bp)
    1767
  • Promoter T7lac
  • Tag / Fusion Protein
    • 6xHis-STag (N terminal on insert)

Cloning Information

  • Cloning method Gene Synthesis
  • 5′ sequencing primer T7 promoter, forward primer
  • 3′ sequencing primer T7 terminator, reverse primer
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT.cruzi-RBP-MASP_6xHis was a gift from Rick Tarleton (Addgene plasmid # 256075 ; http://n2t.net/addgene:256075 ; RRID:Addgene_256075)

  • For your References section:

    Prophylactic low-dose, bi-weekly benznidazole treatment fails to prevent Trypanosoma cruzi infection in dogs under intense transmission pressure. Bustamante JM, Padilla AM, White B, Auckland LD, Busselman RE, Collins S, Malcolm EL, Wilson BF, Saunders AB, Hamer SA, Tarleton RL. PLoS Negl Trop Dis. 2022 Oct 31;16(10):e0010688. doi: 10.1371/journal.pntd.0010688. eCollection 2022 Oct. 10.1371/journal.pntd.0010688 PubMed 36315597
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