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pT.cruzi- p25a-G10_6xHis
(Plasmid #256088)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 256088 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pRSET-TriEX
  • Backbone size w/o insert (bp) 2924
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use BL21 (DE3) for expression
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p25-alpha, putative
  • Alt name
    Tc00.1047053506635.130
  • Alt name
    G10
  • Species
    Trypanosoma cruzi
  • Insert Size (bp)
    459
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis-T7Tag-XpressTag (N terminal on insert)

Cloning Information

  • Cloning method Gene Synthesis
  • 5′ sequencing primer T7 promoter, forward primer
  • 3′ sequencing primer T7 terminator, reverse primer
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's sequencing results differ from the partial sequence information provided by the depositing laboratory, finding several discrepancies in p25a-G10 (M1del, V81A, C145R compared to the reference sequence). The depositor confirms that this change is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT.cruzi- p25a-G10_6xHis was a gift from Rick Tarleton (Addgene plasmid # 256088 ; http://n2t.net/addgene:256088 ; RRID:Addgene_256088)

  • For your References section:

    Prophylactic low-dose, bi-weekly benznidazole treatment fails to prevent Trypanosoma cruzi infection in dogs under intense transmission pressure. Bustamante JM, Padilla AM, White B, Auckland LD, Busselman RE, Collins S, Malcolm EL, Wilson BF, Saunders AB, Hamer SA, Tarleton RL. PLoS Negl Trop Dis. 2022 Oct 31;16(10):e0010688. doi: 10.1371/journal.pntd.0010688. eCollection 2022 Oct. 10.1371/journal.pntd.0010688 PubMed 36315597
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