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pMF2-rSQR
(Plasmid #256132)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 256132 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pMF2
  • Total vector size (bp) 4870
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The S1030 strain (Liu lab, Addgene #105063) should be used for expression. Use of S1030 permits titratable arabinose induction.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sqr
  • Species
    Rhodobacter capsulatus
  • Insert Size (bp)
    1283
  • Mutation
    Codon harmonized for E. coli expression
  • Promoter pBAD

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMF2-rSQR was a gift from Benjamin Woolston (Addgene plasmid # 256132 ; http://n2t.net/addgene:256132 ; RRID:Addgene_256132)

  • For your References section:

    Development of a Transcriptional Biosensor for Hydrogen Sulfide That Functions under Aerobic and Anaerobic Conditions. Fernez MT, Hegde S, Hayes JA, Hoyt KO, Carrier RL, Woolston BM. ACS Synth Biol. 2025 Jun 20;14(6):2198-2207. doi: 10.1021/acssynbio.5c00124. Epub 2025 May 13. 10.1021/acssynbio.5c00124 PubMed 40358934
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