pIVT-TOMM20-mScarlet3
(Plasmid
#256174)
-
PurposeMaking mRNA for TOMM20-mScarlet3
-
Depositing Lab
-
Depositing OrganizationHHMI, Janelia Research Campus
Located in United States of America -
Publication
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 256174 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepIVT
-
Vector typeIVT mRNA expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameTOMM20-mScarlet3
-
SpeciesH. sapiens (human)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.64898/2026.01.02.697412 for bioRxiv preprint. Due to the difficulties of maintaining and sequencing long homopolymers, Addgene cannot guarantee the exact length of polyA sequence. The depositor noted that variations do not impact the function of these plasmids as scientists can re-add all polyA via primers when they PCR the backbone.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pIVT-TOMM20-mScarlet3 was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 256174 ; http://n2t.net/addgene:256174 ; RRID:Addgene_256174) -
For your References section:
mRNAbow: A versatile gene expression system for multiplexed fluorescent imaging using optimized in vitro transcribed mRNA. Choi H, Halanych C, Kasberg W, Testa MD, Rubin-Elgressy S, Nguyen P, Walpita D, Tsang A, Cortes D, Song EY, Wu H, Weissman IL, Espinosa-Medina I, Satou C, Song JL, Matus DQ, Lippincott-Schwartz J. bioRxiv 2026.01.02.697412 10.64898/2026.01.02.697412