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pAAV-nEF-Con/Foff taCasp3-TEVp
(Plasmid #256274)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 256274 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pAAV-nEF-Con/Foff 2.0-Arch3.3-EYFP (Addgene #137149)
  • Backbone manufacturer
    Addgene
  • Vector type
    Mouse Targeting, AAV, Cre/Lox ; INTRSECT

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    INTRSECT taCasp3-TEVp
  • Species
    Synthetic
  • Mutation
    None
  • Promoter nEF

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.07.07.663227 for bioRxiv preprint. 5' cloning site: NcoI (destroyed during cloning), 3' cloning site: NheI (destroyed during cloning).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-nEF-Con/Foff taCasp3-TEVp was a gift from Jean-François Poulin (Addgene plasmid # 256274 ; http://n2t.net/addgene:256274 ; RRID:Addgene_256274)

  • For your References section:

    Calbindin stratifies midbrain dopaminergic neurons governing distinct aspects of locomotion. Bolduc C, Oram C, Donovan S, Bach H, Liu M, Marier R, Sharpe M, Liu S, Campeau C, Spencer CD, Martin SA, Awatramani R, Poulin JF. Proc Natl Acad Sci U S A. 2026 Sep;123(35):e2613593123. doi: 10.1073/pnas.2613593123. Epub 2026 Aug 24. 10.1073/pnas.2613593123 PubMed 42636380
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