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pETDuet-1-PX-Gal-3-TRUNC
(Plasmid #256297)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 256297 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET-Duet-1
  • Backbone size w/o insert (bp) 5420
  • Total vector size (bp) 6580
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PX-Gal-3-TRUNC
  • Species
    H. sapiens (human)
  • Mutation
    Deleted residues 1-116
  • Entrez Gene
    LGALS3 (a.k.a. CBP35, GAL3, GALBP, GALIG, L31, LGALS2, MAC2)
  • Tag / Fusion Protein
    • His tag (N terminal on backbone)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    purchased from Genscript

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETDuet-1-PX-Gal-3-TRUNC was a gift from Mia Huang (Addgene plasmid # 256297 ; http://n2t.net/addgene:256297 ; RRID:Addgene_256297)

  • For your References section:

    Mapping glycan-mediated galectin-3 interactions by live cell proximity labeling. Joeh E, O'Leary T, Li W, Hawkins R, Hung JR, Parker CG, Huang ML. Proc Natl Acad Sci U S A. 2020 Nov 3;117(44):27329-27338. doi: 10.1073/pnas.2009206117. Epub 2020 Oct 16. 10.1073/pnas.2009206117 PubMed 33067390
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