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pLigify_4Hydroxybenzoate
(Plasmid #256300)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 256300 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pTWIST_Kan_Medium
  • Backbone manufacturer
    Twist Biosciences
  • Backbone size w/o insert (bp) 2036
  • Total vector size (bp) 3830
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    4-Hydroxybenzoate-responsive transcription factor
  • Alt name
    HosA
  • Species
    Synthetic; Escherichia coli
  • Insert Size (bp)
    408
  • GenBank ID
    WP_000175365.1

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer atcttaatcatgcgccggtgttttaaat
  • 3′ sequencing primer TAATCCTAATCACTTTCAGCCAAAAAACTTAAGACC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.10.20.683484 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLigify_4Hydroxybenzoate was a gift from Simon d'Oelsnitz & Pamela Silver (Addgene plasmid # 256300 ; http://n2t.net/addgene:256300 ; RRID:Addgene_256300)

  • For your References section:

    Ligify 2.0: A web server for predicted small molecule biosensors. d’Oelsnitz S, Zhao N, Talla P, Jeong J, Love JD, Springer M, Silver PA. bioRxiv 2025.10.20.683484 10.1101/2025.10.20.683484
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