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pASK-CeCav
(Plasmid #256356)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 256356 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pASK-IBA3plus
  • Backbone manufacturer
    IBA GMBH
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Caveolin
  • Species
    C. elegans (nematode)
  • Entrez Gene
    cav-1 (a.k.a. CELE_T13F2.8)
  • Promoter tetracycline promoter/operator
  • Tag / Fusion Protein
    • MBP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pASK-CeCav was a gift from Thierry Galli (Addgene plasmid # 256356 ; http://n2t.net/addgene:256356 ; RRID:Addgene_256356)

  • For your References section:

    A synthetic organelle approach to probe SNARE-mediated membrane fusion in a bacterial host. Ferreras S, Singh NP, Le Borgne R, Bun P, Binz T, Parton RG, Verbavatz JM, Vannier C, Galli T. J Biol Chem. 2023 Mar;299(3):102974. doi: 10.1016/j.jbc.2023.102974. Epub 2023 Feb 3. 10.1016/j.jbc.2023.102974 PubMed 36738791
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