pET28a(+)-LMNA-ThrHis-Cter
(Plasmid #256366)

• Purpose: Human full length lamin A (LMNA) with C-terminal 6x-His tag and cleavable thrombin side. For expression in E. coli ArcticExpress at 24 h, 11-15°C.
• Depositing Lab: Andrei Lupas
• Depositing Organization: Max Planck Institute for Biology
  Located in Germany
• Publication: Lamin A human (full protein) (unpublished)

Backbone

• Vector backbone: pET28a(+)
• Backbone manufacturer: Alexandra Waldherr (Max Planck), BioCat (Heidelberg, Germany)
• Backbone size w/o insert (bp): 5188
• Total vector size (bp): 7219
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Best to culture for expression in ArcticExpress at 13°C O/N.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: LMNA
• Alt name: Lamin A
• Alt name: Lamin A/C
• Alt name: LMNA/C
• Species: H. sapiens (human)
• Insert Size (bp): 2031
• Entrez Gene: LMNA (a.k.a. CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1)
• Promoter: T7
• Tag / Fusion Protein:
  • His-tag: C-terminal which can be cleaved with thrombin digest

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GAAATTAATACGACTCACTATAGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid cannot be used for commercial purposes. Academic use has to be credited to Alexandra Waldherr.